Yes, most businesses can have people in their tour. The one exception is for medical practices. If you have a medical facility, no people (clients or staff) are allowed to be in the tour.

I usually advise clients that these tours are really meant to showcase the space and when possible, I recommend that we shoot when there are few to no people in your business.
Keep in mind that the way in which the tours are shot, any movement will appear as a blurriness. And legally I am required to blur any faces for privacy reasons.
I feel that keeping the space free of people makes for a better experience for the end user.

However, as with most things…there are exceptions to the rules.
If you want to get creative, I am totally up for it!
I shot a tour for Orbit Media, a web development office. They wanted to show all their developers working at their desks, and instead of me blurring their faces out, they created their own newspapers that the developers held up in front of their faces.
Here are some screenshots of their tour and here’s the link to the tour.

Screen Shot of Orbit Media Studio virtual tour

Orbit Media developers getting creative

Screen Shot of Orbit Media Studio virtual tour

Orbit Media developers getting creative